| 货号 | 72096S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Enzymes of the phosphodiesterase (PDE) superfamily catalyze the hydrolysis of 3,5-cyclic nucleotides into the corresponding nucleotide 5-monophosphates. The PDE superfamily includes 11 subfamilies (PDE1-PDE11) in mammals (1). These enzymes function as important positive and negative regulators of cellular response, including regulation of insulin secretion, heart function, erectile function, and inflammation (2-5). The cAMP-specific phosphodiesterase 4B (PDE4B, DPDE4) is important for the inflammatory response to lipopolysaccharide in monocytes (6). PDE4B plays an important role in the hydrolysis and inactivation of the ubiquitous second messenger cAMP that regulates lymphocyte cell growth and apoptosis (7). Research studies indicate that PDE4B is also involved in psychiatric disorders, including schizophrenia, autism, and depression (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from mouse brain, rat brain, and A-204 cells using PDE4B (D7R1Y) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PDE4B protein (hPDE4B-Myc/DDK; +) or Myc/DDK-tagged full-length human PDE4A protein (hPDE4A-Myc/DDK; +), using PDE4B (D7R1Y) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Immunoprecipitation of PDE4B from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PDE4B (D7R1Y) Rabbit mAb. Western blot analysis was performed using PDE4B (D7R1Y) Rabbit mAb. |
