| 货号 | 76194S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB |
| 目标/特异性 | FAT10 (D1Q3Y) Rabbit mAb recognizes endogenous levels of total FAT10 protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | HLA-F adjacent transcript 10 (FAT10/Ubiquitin D) belongs to the ubiquitin-like modifier (Ubl) family of proteins. The 18 kDa FAT10 protein contains two tandem Ubl domains that are oriented in a head-to-tail fashion and a free C-terminal di-glycine motif, which is available for isopeptide bond formation with target proteins via an E1-E2-E3 enzymatic cascade (1). Indeed, FAT10 provides a ubiquitin-independent signal for proteasomal degradation (2). Research studies have demonstrated that FAT10 expression is enriched in lymphoid organs and that its expression is transiently upregulated via the NF-kB pathway in response to pro-inflammatory cytokines such as TNFα and IFNγ (1,3-5). In solid tumors that possess inflammatory microenviroments, research studies have shown that FAT10 is overexpressed and may serve as a biomarker for inflamed tumors (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
| 参考文献 | 1 . Raasi, S. et al. (1999) Eur J Immunol 29, 4030-6. 2 . Hipp, M.S. et al. (2005) Mol Cell Biol 25, 3483-91. 3 . Lee, C.G. et al. (2003) Oncogene 22, 2592-603. 4 . Lukasiak, S. et al. (2008) Oncogene 27, 6068-74. 5 . Ren, J. et al. (2011) J Cell Sci 124, 3665-75. 6 . Lukasiak, S. et al. (2008) Oncogene 27, 6068-74. 7 . Lee, C.G. et al. (2003) Oncogene 22, 2592-603. |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human FAT10 protein (hFAT10-Myc/DDK; +), using FAT10 (D1Q3Y) Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from SW620 and Huh7 cells, untreated (-) or treated (+) with combinations of the following treatments as indicated: hIFNγ (100ng/mL, 16 hr) and hTNFα #8902 (100ng/mL, 16 hr), using FAT10 (D1Q3Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
