| 货号 | 3501S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | PDI (C81H6) Rabbit mAb detects endogenous levels of total PDI protein. |
| 使用方法 | WB(1:1000) IHC-P (1:1250) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3). |
| 存放说明 | -20C |
| 计算分子量 | 57 |
Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb. 对多个细胞系使用PDI (C81H6) Rabbit mAb兔单抗进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb. 对石蜡包埋的人肺癌使用PDI (C81H6) Rabbit mAb兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb. 对石蜡包埋的人淋巴瘤使用PDI (C81H6) Rabbit mAb兔单抗进行免疫组化分析。 | |
Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对NIH/3T3细胞使用PDI (C81H6) Rabbit mAb兔单抗（绿色）和β-Actin (8H10D10) Mouse mAb #3700（红色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb. 对石蜡包埋的小鼠脾脏使用PDI (C81H6) Rabbit mAb兔单抗进行免疫组化分析。 |
