| 货号 | 5662S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PDK1 (D37A7) Rabbit mAb detects endogenous levels of total PDK1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis. |
| 存放说明 | -20C |
| 计算分子量 | 58-68 |
Western blot analysis of extracts from various cell lines using PDK1 (D37A7) Rabbit mAb. Western blot 分析不同细胞系提取物，所用抗体为PDK1 (D37A7) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from HCT 116 wild-type and HCT 116 PDK1-/- cells using PDK1 (D37A7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT 116 wild-type and HCT 116 PDK1-/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD). Western blot分析HCT 116野生型和HCT 116 PDK1 -/-细胞提取物，所用抗体为PDK1 (D37A7) Rabbit mAb兔单抗 (上) 和 Akt (pan) (C67E7) Rabbit mAb 兔单抗#4691 (下)。（ HCT 116野生型和HCT 116 PDK1 -/-细胞由Johns Hopkins University, Baltimore, MD的Bert Vogelstein博士友情提供）。 |
