| 货号 | 13091S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PEA-15 (D1G6A) Rabbit mAb recognizes endogenous levels of total PEA-15 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as in tissues, including the lung and eye (1). The protein has been shown to coordinate cell growth, death, and glucose utilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and further regulates the Erk and apoptosis signaling pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 15 |
Western blot analysis of extracts from various cell lines using PEA-15 (D1G6A) Rabbit mAb. 使用PEA-15 (D1G6A) Rabbit mAb 兔单抗对多种细胞提取物进行 western blot 分析。 | |
Immunoprecipitation of PEA-15 from SK-MEL-28 cell extracts using Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 2) or PEA-15 (D1G6A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PEA-15 (D1G6A) Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 PEA-15 (D1G6A) Rabbit mAb (lane 3)对SK-MEL-28细胞提取物中的PEA-15蛋白进行免疫沉淀实验。Lane1是10%上样量。使用PEA-15 (D1G6A) Rabbit mAb 兔单抗进行western blot分析。 |
