| 货号 | 13045S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | PFKFB2 (D7G5R) Rabbit mAb recognizes endogenous levels of total PFKFB2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). Research studies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2 (3). In addition, androgen increases the expression of PFKFB2 in prostate cancer cells (4). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Confocal immunofluorescent analysis of LNCaP (high expression, left) and Hep G2 (low expression, right) cells using PFKFB2 (D7G5R) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用PFKFB2 (D7G5R) Rabbit mAb兔单抗 (绿色)对LNCaP()高表达，左)和Hep G2(低表达，右)细胞提取物进行激光共聚焦免疫荧光分析。蓝色伪彩= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Western blot analysis of extracts from LNCaP, Hep G2, and MCF7 cells using PFKFB2 (D7G5R) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). 使用PFKFB2 (D7G5R) Rabbit mAb兔单抗 (上) 和 β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下)对LNCaP, Hep G2, 和MCF7细胞提取物进行western blot分析。 | |
Immunoprecipitation of PFKFB2 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PFKFB2 (D7G5R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PFKFB2 (D7G5R) Rabbit mAb. An anti-rabbit IgG light chain antibody was used as the secondary antibody. 使用Rabbit (DA1E) mAb兔单抗 IgG XP® Isotype Control #3900 (lane 2) 或 PFKFB2 (D7G5R) Rabbit mAb兔单抗 (lane 3)对Hep G2细胞提取物中的PFKFB2进行免疫沉淀实验。Lane1是10%上样量。使用PFKFB2 (D7G5R) Rabbit mAb兔单抗进行western blot分析。anti-rabbit IgG light chain antibody被用作二抗。 |
