| 货号 | 13123S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PFKFB3 (D7H4Q) Rabbit mAb recognizes endogenous levels of total PFKFB3 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). One of them, PFKFB3/iPFK-2, was shown to be inducible by hypoxia leading to increased glycolysis under hypoxic conditions (2). Research studies have shown that PFKFB3/iPFK-2 is also highly expressed in some types of human cancer (3). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from various cell lines using PFKFB3 (D7H4Q) Rabbit mAb. 使用PFKFB3 (D7H4Q) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human PFKFB3 (hPFKFB3-DDK; +), using PFKFB3 (D7H4Q) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). 模拟转染(-)或转染了表达DDK标记的full-length human PFKFB3 (hPFKFB3-DDK; +)的293细胞提取物，使用PFKFB3 (D7H4Q) Rabbit mAb兔单抗 (上), DYKDDDDK Tag (9A3) Mouse mAb鼠单抗 #8146 (中), 或 β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下)进行western blot分析。 |
