| 货号 | 12746S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | PFKP (D2E5) Rabbit mAb recognizes endogenous levels of total PFKP protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). There are three isozymes: muscle-type, liver-type, and platelet-type (2,3). Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (4,5). Research studies have shown that genetic variations in PFKP are associated with individuals born small for gestational age that are prone to obesity and diabetes later in adulthood (6). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Confocal immunofluorescent analysis of 293 cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (left) or a PFKP-specific siRNA (right), using PFKP (D2E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析293细胞，用SignalSilence® Control siRNA (Unconjugated) #6568 （左）或一种 PFKP-特异 siRNA （右）转染细胞。使用抗体为PFKP (D2E5) Rabbit mAb兔单抗（绿色）。DY-554 phalloidin（红色）标记肌动蛋白微丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Western blot analysis of extracts from various cell lines using PFKP (D2E5) Rabbit mAb. Wetern blot方法检测多种细胞系提取物，所用抗体为PFKP (D2E5) Rabbit mAb兔单抗。 | |
Immunoprecipitation of PFKP from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PFKP (D2E5) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PFKP (D2E5) Rabbit mAb. 免疫沉淀方法检测HeLa细胞提取物PFKP蛋白，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或PFKP (D2E5) Rabbit mAb兔单抗 (泳道3)。泳道1为10%对照。使用PFKP (D2E5) Rabbit mAb 兔单抗进行Western blot分析。 | |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® PFKP siRNA (+), using PFKP (D2E5) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457. Western blot方法检测293T 细胞提取物，用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或SignalSilence® PFKP siRNA I (+) 转染，使用的抗体为PFKP (D2E5) Rabbit mAb兔单抗 或β-Actin (D6A8) Rabbit mAb 兔单抗#8457。 |
