| 货号 | 14085S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The prohibitins PHB1 and PHB2 are highly conserved, multifunctional proteins present in eukaryotic nuclear and mitochondrial compartments (1). Prohibitin-2 (PHB2, REA) was originally identified as an estrogen receptor-specific coregulator. PHB2 directly interacts with hormone-bound estrogen receptor and represses its transcriptional activity through competitive inhibition of Src-1 coactivation of the estrogen receptor (2,3). Together with COUP transcription factors, PHB2 interacts with histone deacetylases HDAC1 and HDAC5 to mediate transcriptional regulation by the estrogen receptor through coupling the deacetylase to the transcription activation complex (4). Prohibitin PHB1/PHB2 heterodimers form large ring complexes on the mitochondrial membrane (5) and act as chaperones to stabilize mitochondrial proteins, such as OPA1 and Hax1, to support mitochondrial morphogenesis and protect against apoptosis (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 33 |
Confocal immunofluorescent analysis of HT-1080 cells using PHB2 (E1Z5A) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using PHB2 (E1Z5A) Rabbit mAb. | |
Immunoprecipitation of PHB2 from HeLa cell extracts using using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PHB2 (E1Z5A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PHB2 (E1Z5A) Rabbit mAb. |
