| 货号 | 24633S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse, Rat |
| 应用 | WB,IP,IF |
| 目标/特异性 | Puma (D7L9L) Rabbit mAb (Rodent Specific) recognizes endogenous levels of total Puma protein in mouse and rat. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) Immunofluorescence (Immunocytochemistry) (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cytochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suppression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4). |
| 存放说明 | -20C |
| 计算分子量 | 23-25 |
| 参考文献 | 1 . Yu, J. et al. (2001) Mol Cell 7, 673-82. 2 . Nakano, K. and Vousden, K.H. (2001) Mol Cell 7, 683-94. 3 . Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74. 4 . Jeffers, J.R. et al. (2003) Cancer Cell 4, 321-8. 5 . Hemann, M.T. et al. (2004) Proc Natl Acad Sci U S A 101, 9333-8. 6 . Yu, J. et al. (2003) Proc Natl Acad Sci U S A 100, 1931-6. 7 . Villunger, A. et al. (2003) Science 302, 1036-8. |
Western blot analysis of extracts various cell lines using Puma (D7L9L) Rabbit mAb (Rodent Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse Puma (mPuma; +) using Puma (D7L9L) Rabbit mAb (Rodent Specific). | |
Immunoprecipitation of Puma from BaF3 cell extracts. Lane 1 represents 10% input, lane 2 is immuoprecipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Puma (D7L9L) Rabbit mAb (Rodent Specific). Western blot was performed using Puma (D7L9L) Rabbit mAb (Rodent Specific). A conformation specific secondary antibody was used to avoid cross reactivity with IgG. | |
Confocal immunofluorescent analysis of BaF3 (high expressing; left) and J744A.1 (low expressing; right) using Puma (D7L9L) Rabbit mAb (green; upper). Mitochondria were co-labeled with COX IV (4D11-B3-E8) Mouse mAb #11967 (red; lower). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
