| 货号 | 15665S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse IgG1 |
| 应用 | W IP IF-IC |
| 目标/特异性 | LAMP1 (D4O1S) Mouse mAb recognizes endogenous levels of total LAMP1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 42 (non-glycosylated), 90-120 (glycosylated) |
| 参考文献 | 1 . Eskelinen, E.L. et al. (2003) Trends Cell Biol 13, 137-45. 2 . Fukuda, M. (1991) J Biol Chem 266, 21327-30. 3 . Kundra, R. and Kornfeld, S. (1999) J Biol Chem 274, 31039-46. 4 . Rohrer, J. et al. (1996) J Cell Biol 132, 565-76. 5 . Huynh, K.K. et al. (2007) EMBO J 26, 313-24. 6 . Eskelinen, E.L. et al. (2004) Mol Biol Cell 15, 3132-45. |
Western blot analysis of extracts from HeLa and A172 cells using LAMP1 (D4O1S) Mouse mAb. | |
Immunoprecipitation of LAMP1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb Isotype Control #5415, and lane 3 is LAMP1 (D4O1S) Mouse mAb. Western blot was performed using LAMP1 (D4O1S) Mouse mAb. | |
Confocal immunofluorescent analysis of A-431 cells, untreated (left) or treated with Chloroquine #14774 (50 μM, overnight; right), using LAMP1 (D4O1S) Mouse mAb (green) and β-Actin (D6A8) Rabbit mAb #8457 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
