| 货号 | 20772S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | DR3 (D4O3X) Rabbit mAb recognizes endogenous levels of total DR3 protein. A band of unknown origin is detected at at 110 kDa in some cell lines. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. |
| 存放说明 | -20C |
| 计算分子量 | 55-60 |
| 参考文献 | 1 . Nagata, S. (1997) Cell 88, 355-65. 2 . Thorburn, A. (2004) Cell Signal 16, 139-44. 3 . Chinnaiyan, A.M. et al. (1996) Science 274, 990-2. 4 . Kitson, J. et al. (1996) Nature 384, 372-5. 5 . Marsters, S.A. et al. (1996) Curr Biol 6, 1669-76. 6 . Bodmer, J.L. et al. (1997) Immunity 6, 79-88. 7 . Screaton, G.R. et al. (1997) Proc Natl Acad Sci U S A 94, 4615-9. 8 . Marsters, S.A. et al. (1998) Curr Biol 8, 525-8. 9 . Kaptein, A. et al. (2000) FEBS Lett 485, 135-41. 10 . Wang, E.C. et al. (2001) Mol Cell Biol 21, 3451-61. 11 . Osawa, K. et al. (2004) Genes Immun 5, 439-43. 12 . Borysenko, C.W. et al. (2005) Biochem Biophys Res Commun 328, 794-9. |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full length human DR3 (hDR3-Myc/DDK; +) using DR3 (D4O3X) Rabbit mAb. | |
Western blot analysis of various cell lines using DR3 (D4O3X) Rabbit mAb (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower). KARPAS cell line source: Dr. Abraham Kapas at the University of Cambridge. |
