| 货号 | 20729S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | MATK/CHK (D2I6U) Rabbit mAb recognizes endogenous levels of total MATK/CHK protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | MATK/CHK (CTK, NTK and HYL) is a non-receptor tyrosine kinase structually and functionally homologous to Csk kinase. The kinase was identified through molecular cloning from multiple tissues by different research groups. Like Csk, MATK/CHK has a N-terminal SH3 domain, followed by an SH2 domain and a C-terminal catalytic kinase domain (1-4). MATK/CHK inhibits Src family members in several different ways. First, it directly phosphorylates the inhibitory C-terminal tyrosine of Src (as well as other Src family members). This induces a Src protein conformational change from the active to inactive state (2,4). Second, it binds directly to activated Src and induces a conformational change to the inactive state (5,6). The SH2 domain of MATK/CHK directly interacts with the phosphorylated tyrosine of activated receptor tyrosine kinases, such as ErbB-2 and c-Kit, to inhibit receptor function (7-9). MATK/CHK negatively regulates tumor cell growth, migration and invasion (10-13). Decreased expression of the protein has been correlated with brain tumors as well as colon cancers in research studies (14-15). |
| 存放说明 | -20C |
| 计算分子量 | 52,56 |
| 参考文献 | 1 . Sakano, S. et al. (1994) Oncogene 9, 1155-61. 2 . Chow, L.M. et al. (1994) Proc Natl Acad Sci U S A 91, 4975-9. 3 . Bennett, B.D. et al. (1994) J Biol Chem 269, 1068-74. 4 . Klages, S. et al. (1994) Proc Natl Acad Sci U S A 91, 2597-601. 5 . Chong, Y.P. et al. (2004) J Biol Chem 279, 20752-66. 6 . Chong, Y.P. et al. (2006) J Biol Chem 281, 32988-99. 7 . Zrihan-Licht, S. et al. (1998) J Biol Chem 273, 4065-72. 8 . Kim, S. et al. (2002) J Biol Chem 277, 36465-70. 9 . Price, D.J. et al. (1997) J Biol Chem 272, 5915-20. 10 . Lee, B.C. et al. (2005) Cancer Res 65, 2840-5. 11 . McShan, G.D. et al. (2002) Int J Oncol 21, 197-205. 12 . Fu, Y. et al. (2006) Int J Oncol 29, 1453-8. 13 . Dokmanovic, M. et al. (2014) Cancer Biol Ther 15, 1029-41. 14 . Zhu, S. et al. (2008) Oncogene 27, 2027-34. 15 . Kim, S.O. et al. (2004) Cancer 101, 1018-27. |
Immunoprecipitation of MATK/CHK protein from GDM-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MATK/CHK (D2I6U) Rabbit mAb. Western blot analysis was performed using MATK/CHK (D2I6U) Rabbit mAb | |
Western blot analysis of extracts from GDM-1, HL-60 and CMK cell lines using MATK/CHK (D2I6U) Rabbit mAb. |
