| 货号 | 23499S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-F IF-IC |
| 目标/特异性 | POMC (D3R1U) Rabbit mAb recognizes endogenous levels of total POMC protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Pro-Opio-Melano-Cortin (POMC) is a precursor protein expressed in the pituitary and the brain where it is processed into several peptide hormones and neuropeptides. Among these peptides are ACTH, α- and β-MSH, β-and γ-LPH, CLIP, β-endorphin, and N-POMC (1). POMC is involved in hypothalamic circuits regulating feeding behavior and POMC-producing neurons promote satiety (2). POMC neurons are also the target of leptin and insulin for the promotion of the browning of white fat (3). |
| 存放说明 | -20C |
| 计算分子量 | 10, 12, 28, 32 |
| 参考文献 | 1 . Chretien, M. and Mbikay, M. (2016) J Mol Endocrinol. 2 . Yang, Y. et al. (2011) Cell 146, 992-1003. 3 . Dodd, G.T. et al. (2015) Cell 160, 88-104. |
Confocal immunofluorescent analysis of the hypothalamic region of normal mouse brain at 20x (upper) and 60x (lower) magnification using POMC (D3R1U) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of AtT-20 (left) and NIH/3T3 cells (right) using POMC (D3R1U) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from AtT-20 and NIH/3T3 cells using POMC (D3R1U) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
