| 货号 | 28439S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Hamster/Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | Cdc2 (E1Z6R) Rabbit mAb recognizes endogenous levels of total cdc2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5). |
| 存放说明 | -20C |
| 计算分子量 | 34 |
| 参考文献 | 1 . Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001. 2 . Norbury, C. et al. (1991) EMBO J 10, 3321-9. 3 . McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85. 4 . Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71. 5 . Hunter, T. (1995) Cell 80, 225-36. |
Western blot analysis of extracts from various cell lines using cdc2 (E1Z6R) Rabbit mAb. | |
Immunoprecipitation of cdc2 from Jurkat cell extracts. Lane 1 is 5% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is cdc2 (E1Z6R) Rabbit mAb. Western blot was performed using cdc2 (E1Z6R) Rabbit mAb. |
