| 货号 | 12402S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PI3 Kinase Class II α (D3Q5B) Rabbit mAb recognizes endogenous levels of total PI3K class II α protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities (1,2). PI3K Class II α generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate (3). PI3K Class II α is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles (1,4,5). Research studies have indicated that PI3K Class II α regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network (5,6). PI3K Class II α also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin (7). PI3K Class II α has also been shown to regulate neurosecretory granule exocytosis (8) and vascular smooth muscle contraction (9). Unlike other PI3K family members, PI3K Class II α is less sensitive to the PI3K inhibitors wortmannin and LY294002 (3). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
Western blot analysis of extracts from SW620 and HeLa cells using PI3 Kinase Class II α (D3Q5B) Rabbit mAb. 使用PI3 Kinase Class II α (D3Q5B)Rabbit mAb兔单抗对SW620和HeLa细胞提取物进行western blot分析。 | |
