| 货号 | 4249S |
| 反应种属 | Human/Mouse/Rat/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | PI3 Kinase p110α (C73F8) Rabbit mAb detects endogenous levels of total PI3K p110α protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb. Western blot分析HeLa细胞和新生小鼠大脑的提取物，所使用的抗体是I3 Kinase p110α (C73F8) Rabbit mAb兔单抗。免疫组化分析石蜡包埋的人类乳腺癌组织，所使用的是PI3 Kinase p110α (C73F8) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组化分析石蜡包埋的人类肺癌组织，是在对照肽段（左图）和抗原特异性肽段（右图）下应用PI3 Kinase p110α (C73F8) Rabbit mAb兔单抗标记。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb. |
