| 货号 | 11889S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PI3 Kinase p55 (D2B3) Rabbit mAb recognizes endogenous levels of total PI3 kinase p55 (PIK3R3) protein. This antibody does not cross-react with PI3 kinase p85α (PIK3R1) or PI3 kinase p85β (PIK3R2) proteins. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Western blot analysis of extracts from MDA-MB-453, Hs578T, and ZR-75-1 cells using PI3 Kinase p55 (D2B3) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). 使用PI3K激酶p55（D2B3）Rabbit mAb兔单抗（上）或β-Actin (D6A8)Rabbit mAb兔单抗#8457（下）对MDA-MB-453, Hs578T,和ZR-75-1细胞提取物进行western blot分析。 |
