| 货号 | 33942S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | SWAP70 (D5O6A) Rabbit mAb recognizes endogenous levels of total SWAP70 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SWAP70 is a Rac family guanine nucleotide exchange factor (GEF) (1). It is highly expressed by activated B cells. Following B cell stimulation, SWAP70 has been observed to translocate from the cytoplasm to the nucleus, where it plays a role in class switching, as well as to the membrane, where it associates with the B cell receptor (2,3). SWAP70 also plays a role in migration of B cells and other immune cell types including dendritic cells, eosinophils, and mast cells (4-7). Mice deficient in both SWAP70 and a related protein, DEF6, develop lupus-like autoimmunity due to misregulation of IRF4 in B cells and T cells leading to increased IL21 production and responsiveness (8). |
| 存放说明 | -20C |
| 计算分子量 | 70, 74 |
| 参考文献 | 1 . Shinohara, M. et al. (2002) Nature 416, 759-63. 2 . Borggrefe, T. et al. (1998) J Biol Chem 273, 17025-35. 3 . Masat, L. et al. (2000) Proc Natl Acad Sci U S A 97, 2180-4. 4 . Sivalenka, R.R. and Jessberger, R. (2004) Mol Cell Biol 24, 10277-88. 5 . Pearce, G. et al. (2006) Nat Immunol 7, 827-34. 6 . Ocaña-Morgner, C. et al. (2011) J Immunol 186, 5345-55. 7 . Bahaie, N.S. et al. (2012) J Immunol 188, 1479-90. 8 . Biswas, P.S. et al. (2012) J Exp Med 209, 581-96. |
Western blot analysis of extracts from various cell lines using SWAP70 (D5O6A) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SWAP70 (hSWAP70-Myc/DDK; +), using SWAP70 (D5O6A) Rabbit mAb. |
