PICH (D4G8) Rabbit mAb
货号：
8886S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 8886S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | PICH is a helicase of the SNF2 family of ATPases and is essential for proper chromosome segregation during mitosis (1). While PICH was originally proposed to participate in spindle assembly checkpoint signaling (1), that function was subsequently called into question (2). When phosphorylated at Thr1063 by CDK1, PICH binds the polo-box domain of the mitotic kinase PLK1 (1) and targets it to chromosome arms (3), where it appears to facilitate proper chromosome arm cohesion (4). PICH is also a substrate of PLK1 (1). Localized to the cytoplasm during interphase, PICH begins to accumulate at centromeres and kinetochores in prometaphase (4). As chromosomes begin to separate at the onset of anaphase, PICH associates with ultrafine threads between sister centromeres thought to be composed of entangled DNA (5), a natural consequence of DNA replication. PICH is proposed to cooperate with BLM, a RecQ-like helicase implicated in the genetic disorder Bloom’s Syndrome, to displace centromeric histones along these threads, thus enabling them to span large distances without breaking (6). This provides a temporal window for topoisomerase IIα-mediated disentanglement (7). Defects in PICH or BLM disrupt proper chromatid segregation and result in the formation of micronuclei (6).PICH是ATP酶SNF2家族的一个解旋酶，对于有丝分裂过程中适当的染色体分离必不可少（1）。 PICH最初被提出参与纺锤体装配检验点信号（1），该功能随后被质疑（2）。当CDK1磷酸化Thr1063时，PICH结合到有丝分裂激酶PLK1的polo-box结构域（1）并靶向其染色体臂（3），它似乎在那里促进适当的染色体臂凝聚（4）。PICH也是PLK1的底物（1）。在分裂间期间向细胞质聚集，PICH开始积聚在前中期的中心体和着丝粒（4）。由于分裂后期染色体开始分离，PICH联合姐妹中心体之间的超细线程，该结构被认为能够组成DNA（5），缠绕DNA (5)，一种自然的DNA复制的结果。PICH被认为能够合作BLM，一个RecQ样解旋酶，在遗传性疾病布卢姆综合征中有中有重要意义，从而取代沿着这些线程的中心体组蛋白，最终促使他们能够跨越没有断裂的较大距离（6）。这为拓扑异构酶IIα介导的解缠结提供了一个时间窗口（7）。PICH或BLM缺陷可以破坏适当的染色单体分离并导致微核的形成（6）。 |
| 存放说明 | -20C |
| 计算分子量 | 175 |
参考图片Western blot analysis of extracts from various cell lines using PICH (D4G8) Rabbit mAb.Western blot方法检测不同细胞提取物，使用的抗体为PICH (D4G8) Rabbit mAb。 |
