| 货号 | 4165S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Pim-3 (D17C9) Rabbit mAb detects endogenous levels of total Pim-3 protein. It does not cross-react with other Pim family members. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with a mouse Pim-3 construct (+), using Pim-3 (D17C9) Rabbit mAb.Western免疫印迹。用Pim-3 (D17C9) Rabbit mAb研究未转染(-)或转染了鼠源Pim-3质粒的COS-7细胞的细胞提取液。 | |
Western blot analysis of extracts from various cell lines using Pim-3 (D17C9) Rabbit mAb.Western免疫印迹。用Pim-3 (D17C9) Rabbit mAb研究不同细胞系的细胞提取液。 | |
Western blot analysis of recombinant Pim-1, 2, and 3 using Pim-3 (D17C9) Rabbit mAb.Western免疫印迹。用Pim-3 (D17C9) Rabbit mAb研究融合的Pim-1, Pim-2 和Pim-3蛋白。 |
