| 货号 | 12765S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Phosphate transporter 1 (PiT1/SLC20A1) is a sodium dependent phosphate (Pi) transporter that imports Pi into cells. PiT1 was initially identified as a receptor for retroviruses (1,2). It is widely expressed in various tissues where it plays a critical role in maintaining cellular Pi homeostasis (3,4). Phosphate transporter 1 is important in cell proliferation and tumor cell growth independent of PiT1 phosphate transport function (5). Researchers have found that PiT1 is involved in TNF-α induced apoptosis (6). Moreover, phosphate uptake via PiT1 is crucial for vascular calcification (7) and overexpression of PiT1 leads to soft tissue calcification in Werner syndrome patients (8). Additional research indicates that increased PiT1 expression is seen in calcific aortic valve disease (CAVD) tissues, and that PiT1 enhances apoptosis and mineralization by modifying Akt1 levels (9). |
| 存放说明 | -20C |
| 计算分子量 | 75-95 |
Western blot analysis of extracts from various cell lines using PiT1/SLC20A1 (D1Z4X) Rabbit mAb. 使用PiT1/SLC20A1 (D1Z4X) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of PiT1 from HT-1080 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PiT1/SLC20A1 (D1Z4X) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PiT1/SLC20A1 (D1Z4X) Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或PiT1/SLC20A1 (D1Z4X) Rabbit mAb 兔单抗(lane 3)对HT-1080细胞中的PiT1进行免疫沉淀。Lane 1是10%上样量。使用PiT1/SLC20A1 (D1Z4X) Rabbit mAb兔单抗进行western blot分析。 |
