| 货号 | 8188S |
| 反应种属 | Human/Monkey/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | PKD2 (D1A7) Rabbit mAb recognizes endogenous levels of total PKD2 protein. This antibody does not cross react with PKD1 or PKD3. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Protein kinase D2 (PKD2) is one of three members of the protein kinase D family, including PKD1/PKCμ and PKD3/PKCν, that belong to the calcium/calmodulin superfamily of serine/threonine protein kinases (1,2). PKDs contain a conserved, carboxy-terminal catalytic domain, an amino-terminal regulatory region hallmarked by a PH domain that coordinates subcellular localization, and two zinc-finger/C1 lipid-binding domains that mediate activation of the enzyme in response to diacylglycerol (DAG) or phorbol ester (2,3). In addition to lipid-mediated activation, PKD catalytic activity can also be stimulated via phosphorylation of critical serine residues within the activation loop of the enzyme (4-8). Novel PKCs, such as PKCη and PKCε, have been shown to phosphorylate PKD1 at Ser744 and Ser748 (Ser706 and Ser710 in human PKD2), resulting in alleviation of autoinhibition of the enzyme mediated by PH domain interactions with the catalytic domain (5). Phosphorylation and activation of PKD isoforms has also been described for other upstream kinases. For example, casein kinase 2 (CK2) has been shown to phosphorylate PKD2 at Ser244, which promotes nuclear accumulation of PKD2, phosphorylation of HDAC7, and expression of Nur77 (9). Although only a handfull of PKD2 effectors have been identified, PKD2 has been implicated in regulating an array of cellular events, including cell survival, development, growth, migration, and transformation (10-14). PKD2-mediated phosphorylation of at least one known substrate, phosphatidylinositol 4-kinase type IIIβ (PI4KIIIβ), also implicates PKD2 in the formation and regulation of exocytotic transport vesicles from the trans Golgi network (15). |
| 存放说明 | -20C |
| 计算分子量 | 105 |
Confocal immunofluorescent analysis of HT-1080 cells, serum starved (left) or treated with phorbol di-butyrate (PDBu) (100 nM for 15 min) using PKD2 (D1A7) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HT-1080细胞，血清饥饿处理（左）或100nM phorbol di-butyrate（PDBu）处理15分钟（右），使用PKD2 (D1A7)兔单克隆抗体（绿色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of extracts from various cell lines using PKD2 (D1A7) Rabbit mAb. 对多个细胞系使用PKD2 (D1A7)兔单克隆抗体进行Western blot分析。 |
