| 货号 | 5655S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PKD3/PKCν (D57E6) Rabbit mAb recognizes endogenous levels of total PKD3/PKCν protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | PKCν, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation. PKCν is composed of 890 amino acid residues and has 77.3% similarity to human PKCμ (PKCμ) and 77. 4% similarity to mouse PKD (the mouse homolog of PKCμ) (1). The PKCν mRNA is ubiquitously expressed in various tissues. PKCν has two putative diacylglycerol binding C1 domains, suggesting that it may participate in a novel diacylglycerol-mediated signaling pathway (2). PKCν is translocated to the plasma membrane and activated by the diacylglycerol mimic phorbol 12-myristate 13-acetate. PKCν is an important physiologic target of the B-cell receptor (BCR) and exhibits robust activation after BCR engagement (2). GPCR agonists induce a rapid activation of PKCν by a protein kinase C (PKC)-dependent pathway that leads to the phosphorylation of the activation loop of PKCν. PKCν is present both in the nucleus and cytoplasm and this distribution of PKCν results from its continuous shuttling between both compartments by a mechanism that requires a nuclear import receptor and a competent CRM1-nuclear export pathway (3). Cell stimulation with the GPCR agonist neurotensin induces a rapid and reversible plasma membrane translocation of PKCν that is PKC-dependent. |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Western blot analysis of extracts from various cell lines using PKD3/PKCν (D57E6) Rabbit mAb. 对多个细胞系使用PKD3/PKCν (D57E6)兔单克隆抗体进行Western blot分析。 |
