PKG-1α (D10G2) Rabbit mAb
货号：
13511S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 13511S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | <p>Cyclic GMP-dependent kinases (cGK/PKG) belong to the AGC family of serine/threonine protein kinases. In mammals, two genes encode PKG-1 and PKG-2. Alternative PKG-1 splicing yields α and β isoforms, which display tissue-specific expression patterns in humans (1). All PKG family members are activated by increased cellular cGMP, which binds to the enzymes regulatory domain inducing a conformational change and leading to enzyme activation. cGMP levels are increased through the activation of guanylyl cyclases, a process known to occur in part through nitric oxide (NO) signaling (2).</p><p>In addition to well established roles in platelet activation and smooth muscle relaxation (3), PKG signaling is important in many biological processes including cardiac contractility, axon guidance, bone growth, contraction of intestinal smooth muscle, and erectile dysfunction (4).</p>Cyclic GMP依赖性蛋白激酶(cGK/PKG)属于丝氨酸/苏氨酸蛋白激酶AGC家族。在哺乳动物中，两个基因编码PKG-1和PKG-2.PKG-1选择性剪接形成在人体中具有组织特异性表达的α 和β亚型（1）。所有的PKG家族成员都可以被增加的细胞内cGMP水平激活，cGMP与酶的调节结构域结合引发构想改变使酶激活。cGMP水平是通过激活鸟苷酸环化酶而增加的，已经知道该过程部分通过一氧化氮（NO）信号通路调控（2）。除了公认的在血小板激活以及平滑肌松弛过程的作用（3），PKG信号通路对多种生物学过程都非常重要，包括心肌收缩性，轴突引导，骨骼生长，肠平滑肌的收缩，与勃起功能障碍(4)。 |
| 存放说明 | -20C |
| 计算分子量 | 78 |
参考图片Western blot analysis of extracts from 293T and SH-SY5Y cells using PKG-1α (D10G2) Rabbit mAb.使用PKG-1α (D10G2) Rabbit mAb对293T和SH-SY5Y细胞提取物进行western blot分析。 |
Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with constructs expressing full-length human PKG-1α (hPKG-1α; +) or PKG-1β (hPKG-1β; +), using PKG-1α (D10G2) Rabbit mAb (upper) or PKG-1 (C8A4) Rabbit mAb #3248 (lower).使用PKG-1α (D10G2) Rabbit mAb (上)或PKG-1 (C8A4) Rabbit mAb #3248 (下)对模拟转染(-)或转染了表达全长人PKG-1α 质粒(hPKG-1α; +)或PKG-1β 质粒(hPKG-1β; +)的COS-7细胞提取物进行western blot分析。， |
