| 货号 | 12254S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Plectin-1 (D6A11) Rabbit mAb recognizes endogenous levels of total plectin-1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Plectin is a large, widely expressed protein that crosslinks the intermediate filament and actin cytoskeleton, mechanically stabilizing cells and tissues. Plectin also plays a role in the regulation of actin dynamics and acts as a scaffold for signaling molecules (1). Plectin is important in the stabilization of hemidesmosomes, crosslinking them to the intermediate filament network. Research studies have shown that mutations in plectin and other genes coding for hemidesmosomal proteins can cause epidermolysis bullosa, a condition manifested by fragile skin and frequent blistering (1,2). Plectin modulates signals to PKC through binding and sequestration of RACK1, the receptor for activated C kinase 1 (3,4). Plectin is also involved in the regulation of cytokeratin architecture and cell stress response (4), signaling through the chemokine receptor CXCR4 (5) and regulation of AMP-activated protein kinase (AMPK) activity and signaling in mouse myotubes (6). |
| 存放说明 | -20C |
| 计算分子量 | 400-500 |
Confocal immunofluorescent analysis of HeLa cells using Plectin-1 (D6A11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫分析HeLa细胞，使用的抗体是Plectin-1 (D6A11) Rabbit mAb (绿色)。蓝色伪彩= DRAQ5® #4084 (荧光DNA染料）。 | |
Western blot analysis of extracts from COS-7 and U-2 OS cells using Plectin-1 (D6A11) Rabbit mAb.Western blot分析COS-7和U-2 OS细胞提取物，使用的抗体是 Plectin-1 (D6A11) Rabbit mAb。 | |
Immunoprecipitation of plectin-1 from U-2 OS cell extracts, using Plectin-1 (D6A11) Rabbit mAb (lane 2) or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Plectin-1 (D6A11) Rabbit mAb.免疫沉淀分析U-2 OS细胞提取物中的plectin-1，采用的抗体是Plectin-1 (D6A11) Rabbit mAb (lane 2) 或Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 3)。Lane 1的浓度为10% input。Western blot分析采用的抗体是Plectin-1 (D6A11) Rabbit mAb。 |
