| 货号 | 14812S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | At least five distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, PLK4/SAK, and the non-catalytic PLK5 protein (1). The p53-induced PLK2 functions in centriole duplication, as well as at spindle and S phase checkpoints (3-5). Research studies show that PLK2 expression is related to chemosensitivity in ovarian cancer. Downregulation of PLK2 expression in chemosensitive ovarian cancer cells is associated with a greater chance of relapse in patients following specific treatment regimens (6). PLK2 can phosphorylate α-synuclein at Ser129, which is a site shown to be involved in diseases of the central nervous system (7,8). Polo-like kinase 2 also phosphorylates GEFs and GAPs, regulating Ras and Rap small GTPase function in neurons (9). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from HCT 116 cells, untreated (-) or treated with Doxorubicin #5927 (0.5 μM, 24 hr; +), using PLK2 (D5R2B) Rabbit mAb (upper), p53 (7F5) Rabbit mAb #2527 (middle), and β-Actin (13E5) Rabbit mAb #4970 (lower). | |
Immunoprecipitation of PLK2 from ACHN cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PLK2 (D5R2B) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using PLK2 (D5R2B) Rabbit mAb. |
