| 货号 | 9047S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Podoplanin (D9D7) Rabbit mAb recognizes endogenous levels of total podoplanin protein. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | Podoplanin (aggrus, glycoprotein 36) is a single-pass transmembrane protein belonging to the type-1 family of sialomucin-like glycoproteins. Podoplanin was first described in the rat as a surface glycoprotein that regulated podocyte morphology (1). It is now commonly used as a marker of lymphatic endothelial cells, where its expression is associated with the process of lymphangiogenesis (2). Its role in this regard is presumably due to its putative involvement in regulating actin cytoskeleton dynamics (3). Research studies have shown that podoplanin expression is upregulated in a number of tumor types including colorectal cancers (4), oral squamous cell carcinomas (5), and germ cell tumors (6), with higher expression levels often associated with more aggressive tumors (7). Research studies have suggested a functional role for podoplanin in the stromal microenvironment of tumors. For example, it has been reported that podoplanin expression in cancer-associated fibroblasts (CAFs) is positively associated with a stromal environment that promotes cancer progression (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 36 |
Western blot analysis of extracts from various cell lines using Podoplanin (D9D7) Rabbit mAb. 使用Podoplanin (D9D7) Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of podoplanin from U-87 MG cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Podoplanin (D9D7) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Podoplanin (D9D7) Rabbit mAb.使用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或Podoplanin (D9D7) Rabbit mAb（lane3）对U-87 MG细胞中的podoplanin进行免疫沉淀。Lane1是10%上样量。使用Podoplanin (D9D7) Rabbit mAb进行western blot分析。 |
