| 货号 | 12825S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/ChIP |
| 目标/特异性 | POLR3A (D5Y2D) Rabbit mAb recognizes endogenous levels of total POLR3A protein. |
| 使用方法 | WB(1:1000) ChIP (1:50) |
| 供应商 | CST |
| 背景 | POLR3A is the largest subunit of the DNA-dependent RNA polymerase III, one of the DNA-dependent RNA polymerases that transcribe small non-coding RNAs such as the 5S rRNA, tRNAs and some miRNAs (1-3). Together with the second largest subunit, POLR3A forms the catalytic center of the polymerase (4). In addition, RNA polymerase III plays a role in the innate immune response by sensing non-self double stranded DNA. Transcription of the non-self DNA into RNA induces type I interferon production through the RIG-I pathway (5,6). Studies suggest that mutations in the POLR3A gene may be linked to hypomyelinating leukodystrophies; a group of inherited neurodegenerative disorders (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 165 |
Western blot analysis of extracts from various cell lines using POLR3A (D5Y2D) Rabbit mAb.Western blot 方法检测不同细胞系的提取物，使用的抗体为POLR3A (D5Y2D) Rabbit mAb | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of POLR3A (D5Y2D) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human L1M5 LINE element primers, SimpleChIP® Human tRNA-Leu Anti-Codon (TAG) Primers #12769, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用从4 x 106 HeLa细胞中提取的交联过的染色质，以及10 µl POLR3A (D5Y2D) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉。所用试剂盒为SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集到的DNA经过实时PCR定量，使用引物为人L1M5 LINE 元件引物，SimpleChIP® Human tRNA-Leu Anti-Codon (TAG) Primers #12769以及 SimpleChIP® Human α Satellite Repeat Primers #4486.。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。 |
