| 货号 | 15141S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | The 26S proteasome is a highly abundant proteolytic complex involved in the degradation of ubiquitinated substrate proteins. It consists largely of the 20S catalytic core particle (CP) and the 19S/PA700 regulatory particle (RP) that caps either end of the CP. The CP consists of two stacked heteroheptameric β-rings (β1-7) that contain three catalytic β-subunits flanked on either side by two heteroheptameric α-rings (α1-7). The RP includes multimeric base and lid complexes. The RP base includes a heterohexameric ring of ATPase subunits that unfold the substrate and open the α-subunit gate to expose the substrate to the catalytic β-subunits. The lid consists of ubiquitin receptors and DUBs that recruit ubiquitinated substrates and modify ubiquitin chain topology (1,2). Proteasome activity modulators, such as PA28/11S REG, bind the 20S CP cylinder end and open the CP channel (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 16 |
Western blot analysis of extracts from various cell lines using POMP (D2X9S) Rabbit mAb. | |
Immunoprecipitation of POMP from RKO cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or POMP (D2X9S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using POMP (D2X9S) Rabbit mAb. | |
Western blot analysis of extracts from RKO cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 72 hr; +), using POMP (D2X9S) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Interferon-γ induces increased POMP protein expression in RKO cells. |
