| 货号 | 37785S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IHC-P ChIP |
| 目标/特异性 | TFEB (D2O7D) Rabbit mAb recognizes endogenous levels of total TFEB protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:600) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network (1,2). TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the up-regulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy (1,2). TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in up-regulation of autophagosomes and lysosomes (3-5). Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress (6-9). During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes (10). TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB (3). |
| 存放说明 | -20C |
| 计算分子量 | 65-70 |
| 参考文献 | 1 . Sardiello, M. et al. (2009) Science 325, 473-7. 2 . Sardiello, M. and Ballabio, A. (2009) Cell Cycle 8, 4021-2. 3 . Settembre, C. et al. (2011) Science 332, 1429-33. 4 . David, R. (2011) Nat Rev Mol Cell Biol 12, 404. 5 . Cuervo, A.M. (2011) Science 332, 1392-3. 6 . Peña-Llopis, S. et al. (2011) EMBO J 30, 3242-58. 7 . Settembre, C. and Ballabio, A. (2011) Autophagy 7, 1379-81. 8 . Peña-Llopis, S. and Brugarolas, J. (2011) Cell Cycle 10, 3987-8. 9 . Settembre, C. et al. (2012) EMBO J 31, 1095-108. 10 . Martina, J.A. et al. (2012) Autophagy 8, (6):903-14. |
Immunohistochemical analysis of paraffin-embedded human lymphoma using TFEB (D2O7D) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using TFEB (D2O7D) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TFEB (D2O7D) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Raji cells treated with Torin-1 (250 nM, 5 hr) and either 10 μl of TFEB (D2O7D) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GLA Promoter Primers #27131, human ATP6V1H promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunoprecipitation of TFEB from Raji cell extracts. Lane 1 represents 10% input, lane 2 is immunoprecipitated with Rabbit (DA1E) mAb IgG XP® Isotype control #3900, and lane 3 is TFEB (D2O7D) Rabbit mAb. Western blot was performed using TFEB (D2O7D) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expresssing full-length human TFEB (hTFEB; +) using TFEB (D2O7D) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using TFEB (D2O7D) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
