| 货号 | 2443S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5).过氧化物酶体增殖物激活受体γ（PPARγ）是配体激活的核受体超家族成员，作为转录激活剂起作用（1）。 PPARγ优先在脂肪细胞、血管平滑肌细胞和巨噬细胞表达（2）。除此之外，它在介导脂肪细胞分化和脂质代谢中起作用（2），PPARγ调节胰岛素敏感性、细胞增殖和炎症（3）。 PPARγ的转录活性可以被MAP激酶通过磷酸化PPARγ丝氨酸（84位）抑制（4,5）。 |
| 存放说明 | -20C |
| 计算分子量 | 53, 57 |
Confocal immunofluorescent analysis of 3T3-L1 cells, using PPARγ (81B8) Rabbit mAb (red) showing nuclear localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测3T3-L1细胞，使用的抗体为PPARγ (81B8) Rabbit mAb（红色），显示出分化细胞的细胞核位置。用BODIPY 493/503标记脂肪滴（绿色）。蓝色伪彩色为DNA荧光染料（产品信息为DRAQ5® #4084）。 | |
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells (differentiated 6 days) using PPARγ (81B8) Rabbit mAb.Western blot方法检测NIH/3T3细胞和3T3-L1细胞（分化6天）提取物，使用的抗体为PPARγ (81B8) Rabbit mAb. |
