| 货号 | 8499S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Prdx1 (D5G12) Rabbit mAb recognizes endogenous levels of total Prdx1 protein. This antibody does not cross-react with Prdx2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Prdx1 belongs to a family of non-seleno peroxidases that function as H2O2 scavengers. All 6 Prdx isoforms share a conserved N-terminal cysteine (Cys51) that is oxidized by H2O2 to form cysteine-sulfenic acid (Cys51-SOH) and, in turn, reacts with Cys172-SH of another Prdx protein, forming a disulfide dimer and protecting it from degradation (1-3). Abnormally high levels of H2O2 cause Prdx1 to form an oligomeric chaperone that loses its peroxidase activity (4). Prdx family members have been reported to bind to JNK and c-Abl and regulate their kinase acitivity (5,6). Prdx1 was shown to bind to PTEN and regulate its phosphatase activity in conditions of mild or no cellular stress, hence preventing Akt-driven transformation by protecting PTEN from oxidation-induced inactivation (7). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
Western blot analysis of extracts from HeLa and MCF7 cells using Prdx1 (D5G12) Rabbit mAb. 对HeLa和MCF7细胞抽提液使用Prdx1 (D5G12)兔单克隆抗体进行Western blot分析。 |
