| 货号 | 39224S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IHC-P IF-IC |
| 目标/特异性 | Mcl-1 (D5V5L) Rabbit mAb recognizes endogenous levels of total Mcl-1 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:150) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mcl-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway (1). Similar to other Bcl-2 family members, Mcl-1 localizes to the mitochondria (2), interacts with and antagonizes pro-apoptotic Bcl-2 family members (3), and inhibits apoptosis induced by a number of cytotoxic stimuli (4). Mcl-1 differs from its other family members in its regulation at both the transcriptional and post-translational level. First, Mcl-1 has an extended amino-terminal PEST region, which is responsible for its relatively short half-life (1,2). Second, unlike other family members, Mcl-1 is rapidly transcribed via a PI3K/Akt dependent pathway, resulting in its increased expression during myeloid differentiation and cytokine stimulation (1,5-7). Mcl-1 is phosphorylated in response to treatment with phorbol ester, microtubule-damaging agents, oxidative stress, and cytokine withdrawal (8-11). Phosphorylation at Thr163, the conserved MAP kinase/ERK site located within the PEST region, slows Mcl-1 protein turnover (10) but may prime the GSK-3 mediated phosphorylation at Ser159 that leads to Mcl-1 destabilization (11). Mcl-1 deficiency in mice results in peri-implantation lethality (12). In addition, conditional disruption of the corresponding mcl-1 gene shows that Mcl-1 plays an important role in early lymphoid development and in the maintenance of mature lymphocytes (13). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Kozopas, K.M. et al. (1993) Proc Natl Acad Sci USA 90, 3516-20. 2 . Yang, T. et al. (1995) J Cell Biol 128, 1173-84. 3 . Sato, T. et al. (1994) Proc Natl Acad Sci USA 91, 9238-42. 4 . Zhou, P. et al. (1997) Blood 89, 630-43. 5 . Wang, J.M. et al. (1999) Mol Cell Biol 19, 6195-206. 6 . Jourdan, M. et al. (2003) Oncogene 22, 2950-9. 7 . Chao, J.R. et al. (1998) Mol Cell Biol 18, 4883-98. 8 . Domina, A.M. et al. (2000) J Biol Chem 275, 21688-94. 9 . Inoshita, S. et al. (2002) J Biol Chem 277, 43730-4. 10 . Domina, A.M. et al. (2004) Oncogene 23, 5301-15. 11 . Maurer, U. et al. (2006) Mol Cell 21, 749-60. 12 . Rinkenberger, J.L. et al. (2000) Genes Dev 14, 23-7. 13 . Opferman, J.T. et al. (2003) Nature 426, 671-6. |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Mcl-1 (D5V5L) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines, using Mcl-1 (D5V5L) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Mcl-1 (D5V5L) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded MCF7 (left) and SK-OV-3 (right) cell pellets using Mcl-1 (D5V5L) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Mcl-1 (D5V5L) Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right). | |
Confocal immunofluorescent analysis of MCF7 (higher-expressing; left) and SK-OV-3 (lower-expressing; right) cells using MCL1 (D5V5L) Rabbit mAb (green). Actin filaments were labeled with DyLightTM 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
