| 货号 | 40103S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse IgG1 |
| 应用 | W IHC-P |
| 目标/特异性 | Galectin-1/LGALS1 (8A12) Mouse mAb recognizes endogenous levels of total galectin-1 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Galectins are a family of β-galactose binding proteins that are characterized by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and their carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions including cell adhesion (3), growth regulation (4), cytokine production (5), T cell apoptosis (6), and immune responses (7). Galectin-1/LGALS1 has been shown to be expressed in a wide range of tissues and cell types. The level and pattern of expression of galectin-1 have been shown to change during development (8). In addition to a role in developmental processes, galectin-1 has been shown to be involved in central immune tolerance and may function in tumorigenesis by modulating the immune response to the tumor (9,10). Research studies have shown that galectin-1 expression is increased in several human cancers, suggesting a correlation with metastatic potential (10). |
| 存放说明 | -20C |
| 计算分子量 | 15 |
| 参考文献 | 1 . Barondes, S.H. et al. (1994) Cell 76, 597-8. 2 . Barondes, S.H. et al. (1994) J Biol Chem 269, 20807-10. 3 . Offner, H. et al. (1990) J Neuroimmunol 28, 177-84. 4 . Wells, V. and Mallucci, L. (1991) Cell 64, 91-7. 5 . Filer, A. et al. (2009) Arthritis Rheum 60, 1604-14. 6 . Perillo, N.L. et al. (1995) Nature 378, 736-9. 7 . Cooper, D.N. et al. (1991) J Cell Biol 115, 1437-48. 8 . Puche, A.C. et al. (1996) Dev Biol 179, 274-87. 9 . van den Brûle, F. et al. (2003) Lab Invest 83, 377-86. 10 . Salatino, M. et al. (2008) Expert Opin Biol Ther 8, 45-57. |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Galectin-1/LGALS1 (8A12) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded A549 (left) and LNCaP (right) cell pellets using Galectin-1/LGALS1 (8A12) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Galectin-1/LGALS1 (8A12) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human stomach using Galectin-1/LGALS1 (8A12) Mouse mAb. | |
Western blot analysis of extracts from various cell lines using Galectin-1/LGALS1 (8A12) Mouse mAb (upper) or β-Actin (D6A9) #8457 (lower). The LNCap cell line extract is negative for galectin-1/LGALS1 expression as expected. |
