货号 | 12446S |
反应种属 | Human/Mouse/Rat/Monkey |
来源宿主 | Rabbit |
应用 | W/IP |
目标/特异性 | PSMA3 (D4Y9O) Rabbit mAb recognizes endogenous levels of total PSMA3 protein. This antibody does not cross-react with other α subunits of the 20S proteasome. This antibody cross-reacts with a 140 kDa protein of unknown identity in lysates from certain cell lines. |
使用方法 | WB(1:1000) IP (1:50) |
供应商 | CST |
背景 | PSMA3 (HC8/α7) is an α subunit of the 20S proteasome. PSMA3 lacks any known proteolytic activity but may have an important role in the intiation of ring formation and 20S proteasome assembly (3). Research studies have demonstrated that PSMA3 serves as a receptor within the proteasome, recruiting substrates that are destined for degradation in a ubiquitination-independent manner (4,5). |
存放说明 | -20C |
计算分子量 | 28 |
Western blot analysis of extracts from various cell lines using PSMA3 (D4Y9O) Rabbit mAb. Western blot检测多种细胞提取物。 | |
Immunoprecipitation of PSMA3 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PSMA3 (D4Y9O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PSMA3 (D4Y9O) Rabbit mAb. Immunoprecipitation检测293T细胞的PSMA3和Rabbit (DA1E) mAb IgG XP®同型对照#3900 (lane 2)或PSMA3 (D4Y9O) Rabbit mAb (lane 3)。Lane 1中样品浓度为10%。Western blot检测采用PSMA3 (D4Y9O) Rabbit mAb。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMA1 (hPSMA1-Myc/DDK; +), full-length human PSMA2 (hPSMA2-Myc/DDK; +), full-length human PSMA3 (hPSMA3-Myc/DDK; +), full-length human PSMA4 (hPSMA4-Myc/DDK; +), full-length human PSMA6 (hPSMA6-Myc/DDK; +), or full-length human PSMA7 (hPSMA7-Myc/DDK; +), using PSMA3 (D4Y9O) Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).Western blot 检测mock transfected (-)或转染表达Myc/DDK-tagged 人全长PSMA1 (hPSMA1-Myc/DDK; +), 人全长PSMA2 (hPSMA2-Myc/DDK; +),人全长PSMA3 (hPSMA3-Myc/DDK; +), 人全长PSMA4 (hPSMA4-Myc/DDK; +), 人全长PSMA6 (hPSMA6-Myc/DDK; +), 或人全长PSMA7 (hPSMA7-Myc/DDK; +)的293T细胞, 采用PSMA3 (D4Y9O) Rabbit mAb (upper)或DYKDDDDK Tag Antibody #2368 (lower)。 |