PSMB7 (E1L5H) Rabbit mAb
货号：
13207S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 13207S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PSMB7 (E1L5H) Rabbit mAb recognizes endogenous levels of total PSMB7 protein. This antibody reacts with precursor and mature forms of PSMB7, but does not cross-react with PSMB10. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The core particle exhibits three distinct enzymatic activities, each catalyzed by a separate protein subunit. The constitutively expressed PSMB5, PSMB7, and PSMB6 subunits provide chymotrypsin-like, trypsin-like, and caspase-like activities, respectively. These catalytic subunits belong to the amino-terminal nucleophile (Ntn) hydrolase family and are characterized by a single-residue active site. The catalytic β-subunits are synthesized with amino-terminal propeptides, which are removed at the final step of proteasome biogenesis to expose the catalytic threonine residues (3). In immune cells involved in antigen presentation, the constitutively expressed PSMB6, PSMB7, and PSMB5 subunits are replaced by three highly homologous, induced β-subunits to form the immunoproteasome (4,5). PSMB7 is downregulated at the protein level by IFN-γ and replaced by PSMB10 to remodel the proteolytic specificity of the proteasome for more appropriate immunological processing of endogenous antigens (6). Research studies show that PSMB7 expression is upregulated in human colon adenocarcinomas and suggest that high PSMB7 expression may serve as a potential prognostic marker in breast cancer (7,8).核心颗粒由它内部的三种催化活性类型构成：糜蛋白酶样、胰蛋白酶样和半胱天冬酶样活性，由组成性表达的PSMB5 (β5/MB1/X/LMPX/Macropain epsilon链), PSMB7 (β2/Z/Macropain 链 Z)和PSMB6 (β1/Y/LMPY/Macropain delta 链)亚基分别组成。这些催化亚基属于N-末端亲核(Ntn)水解酶家族，并有标志性的单一残基活化位点。每一种蛋白水解亚基的N-末端苏氨酸提供催化的亲核体（在侧链上）和主要的质子接受体（在主要的链N-末端）。β-催化亚基由N-末端前肽合成，通过限制性蛋白水解暴露于催化的苏氨酸残基，在蛋白酶体发生的最后被去除(3)。免疫应答的免疫细胞中，组成性地表达PSMB6、PSMB、PSMB5亚基被3种高度同源的诱导β-亚基：PSMB9 (β1i/LMP2/RING12), PSMB10 (β2i/MECL-1/LMP10) 和 PSMB8 (β5i/LMP7/RING10)分别替代，从而形成免疫蛋白酶体(4,5)。PSMB7由IFN-γ在蛋白水平小调，并由PSMB10/MECL-1取代，为了改变蛋白酶体的蛋白水解特异性，从而为内源性抗原提供适当的免疫处理(6)。研究者已经证实PSMB7的表达在人结肠腺癌中上调，此外，研究表明高表达的PSMB7可能作为乳腺癌愈后标志(7,8)。 |
| 存放说明 | -20C |
| 计算分子量 | 28, 30 |
参考图片Western blot analysis of extracts from various cell lines using PSMB7 (E1L5H) Rabbit mAb.Western blot检测多种细胞提取物，使用的抗体是PSMB7 (E1L5H) Rabbit mAb。 |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 72 hr; +), using PSMB7 (E1L5H) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).Western blot分析HeLa细胞提取物，未处理（-）或人干扰素处理(hIFN-γ) #8901 (100ng/ml, 72小时;+)，使用的抗体是PSMB7 (E1L5H) Rabbit mAb (上图) 和GAPDH (D16H11) XP® Rabbit mAb #5174 (下图)。 |
