PSMC2 (D5T1T) Rabbit mAb
货号：
14395S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 14395S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The base of the eukaryotic proteasome 19S/PA700 RP contains six AAA-ATPase subunits (PSMC1-PSMC6) that bind directly to the 20S CP α-ring. These 19S RP ATPases are thought to assemble into a heterohexameric, pore-like structure that forms part of the substrate translocation channel. Energy derived from ATP hydrolysis by the AAA-ATPases is utilized for substrate unfolding and translocation, which is required for degradation of ubiquitinated folded proteins within the central chamber of the 20S CP formed by β-subunits (3-5). PSMC2 (RPT1, MSS1) is a AAA-ATPase subunit of the 19S/PA700 RP. Research studies have shown that PSMC2 is associated with several components of the basal transcriptional machinery suggesting that PSMC2, in addition to participating in proteasome-dependent degradation of proteins, may also play a role in gene transcription (6). More recently, it has been shown that numerous human cancer cell lines have reduced PSMC2 expression resulting from loss of PSMC2 copy number loss and display a strict threshold requirement for PSMC2 levels in order to sustain a proliferative advantage (7). |
| 存放说明 | -20C |
| 计算分子量 | 47 |
参考图片Western blot analysis of extracts from various cell lines using PSMC2 (D5T1T) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). OVCAR8 cells are reported to express low levels of PSMC2 (7). |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMC1 protein (hPSMC1-Myc/DDK; +), full-length human PSMC2 protein (hPSMC2-Myc/DDK; +), full-length human PSMC3 protein (hPSMC3-Myc/DDK; +), full-length human PSMC4 protein (hPSMC4-Myc/DDK; +), full-length human PSMC5 protein (hPSMC5-Myc/DDK; +), and full-length human PSMC6 protein (hPSMC6-Myc/DDK; +), using PSMC2 (D5T1T) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower). |
