| 货号 | 4197S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PSMD14 (D18C7) Rabbit mAb recognizes endogenous levels of total PSMD14 protein. This antibody does not cross-react with COPS5/JAB1. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Of the subunits that comprise the 19S RP lid, only PSMD14 (POH1) has a known function. PSMD14 is classified as a metalloenzyme DUB and its activity has been shown to be critical for proteasome function in both yeast and humans (3-6). Indeed, PSMD14 harbors an Mpr1-Pad1-N-terminal (MPN) domain with an embedded, highly conserved signature motif for metal-dependent isopeptidases that has been dubbed JAMM, from Jab1/Pad1/MPN domain metalloenzyme. The JAMM motif of PSMD14 consists of a pattern of four charged amino acids: a glutamate residue followed by two histidines and an aspartate (EXnHXHX10D). It has been proposed that the histidine residues, in concert with the aspartate, bind to a zinc ion that, together with the preceding glutamate, forms the catalytic site of PSMD14 (3,4). PSMD14 is thought to cleave ubiquitin chains with proximal specificity relative to the substrate, thus removing whole ubiquitin chains en bloc (3). Recent studies have implicated PSMD14 as an important regulator of ErbB2 (7) and c-Jun (8) protein levels. |
| 存放说明 | -20C |
| 计算分子量 | 34 |
Western blot analysis of extracts from various cell lines using PSMD14 (D18C7) Rabbit mAb. Western blot检测多种细胞提取物。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a cDNA construct expressing Myc/DDK-tagged full-length human PSMD14 (hPSMD14-Myc/DDK, +), using PSMD14 (D18C7) Rabbit mAb. Western blot检测mock transfected (-) 或转染cDNA构成表达Myc/DDK-tagged人全长PSMD14 (hPSMD14-Myc/DDK, +)，采用PSMD14 (D18C7) Rabbit mAb。 |
