| 货号 | 12450S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Puma (D30C10) Rabbit mAb recognizes endogenous levels of total Puma protein. This antibody also cross-reacts with a protein of unknown origin at 60 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cyctochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suprression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Western blot analysis of extracts from various cell lines using Puma (D30C10) Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为Puma (D30C10) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from A549 cells, untreated (-) or treated with Doxorubicin #5927 (500 nM, overnight; +), using Puma (D30C10) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot方法检测 A549 细胞提取物，未经处理(-)或Doxorubicin #5927 (500 nM, 过夜; +)处理，使用抗体为Puma (D30C10) Rabbit mAb 兔单抗（上图）或β-Actin (D6A8) Rabbit mAb 兔单抗#8457（下图）。 | |
Immunoprecipitation of Puma from RL-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Puma (D30C10) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Puma (D30C10) Rabbit mAb. 免疫沉淀方法检测RL-7细胞提取物中的Puma，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或Puma (D30C10) Rabbit mAb 兔单抗(泳道3)，泳道1为10%对照。使用Puma (D30C10) Rabbit mAb 兔单抗进行Western blot分析。 |
