| 货号 | 14570S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cytochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suppression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4). |
| 存放说明 | -20C |
| 计算分子量 | 23-25 |
Western blot analysis of extracts from various cell lines using Puma (E1S7A) Rabbit mAb (Rodent Specific). | |
Immunoprecipitation of Puma from M1 cells using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Puma (E1S7A) Rabbit mAb (Rodent Specific) (lane 3). Lane 1 represents 10% input. Western blot was performed using Puma (E1S7A) Rabbit mAb (Rodent Specific). Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid cross reactivity with IgG light chain. |
