| 货号 | 3205S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Pyruvate Dehydrogenase (C54G1) Rabbit mAb detects endogenous levels of total pyruvate dehydrogenase α1 subunit. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis. The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (1). This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). Pyruvate dehydrogenase (E1) consists of two subunits: α and β. This enzyme catalyzes the removal of CO2 from pyruvate. Mutations in the α subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death (2). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb. 免疫组织化学检测石蜡包埋的人肺癌组织，所用抗体为Pyruvate Dehydrogenase (C54G1) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组织化学检测石蜡包埋的人乳腺癌组织，所用抗体为Pyruvate Dehydrogenase (C54G1) Rabbit mAb兔单抗。左为对照肽，右为抗原肽。 | |
Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb. Wetern blot检测多种细胞提取物，所用抗体为Pyruvate Dehydrogenase (C54G1) Rabbit mAb兔单抗。 | |
Western blot analysis of various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb. Wetern blot检测多种细胞提取物，所用抗体为Pyruvate Dehydrogenase (C54G1) Rabbit mAb兔单抗。 |
