| 货号 | 12849S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Rab11FIP1 (D9D8P) Rabbit mAb recognizes endogenous levels of total Rab11FIP1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Rab11 family proteins consist of closely related Rab11a, Rab11b, and Rab25. They are small GTPases thought to play an essential role in regulating endocytic membrane traffic (1,2). The GTP-bound active state Rab11 proteins interact with the Rab11 family interacting proteins (Rab11FIPs) via a conserved carboxy-terminal Rab11-binding domain (3,4). At least six members of the Rab11FIPs have been identified. Based on sequence homology and domain structures, Rab11FIP1, Rab11FIP2 and Rab11FIP5 are categorized as class I subfamily members, whereas Rab11FIP3 and Rab11FIP4 belong to the class II subfamily that bind Arf6 and Rab11 proteins (5-7). Research studies have implicated Rab11 family proteins and their interacting effectors in carcinogenesis (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 85 |
Confocal immunofluorescent analysis of MCF7 (positive, left) and SNB19 (negative, right) cells using RAB11FIP1 (D9D8P) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用RAB11FIP1 (D9D8P) Rabbit mAb(绿色)对MCF7(阳性，左)和SNB19(阴性，右)细胞进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5®#4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa, Hep G2, and BaF3 cells using Rab11FIP1 (D9D8P) Rabbit mAb.使用Rab11FIP1 (D9D8P) Rabbit mAb对HeLa, Hep G2,和BaF3细胞提取物进行western blot分析。 | |
Immunoprecipitation of Rab11FIP1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Rab11FIP1 (D9D8P) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Rab11FIP1 (D9D8P) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或Rab11FIP1 (D9D8P) Rabbit mAb (lane 3)对HeLa细胞中的Rab11FIP1进行免疫沉淀。Lane 1是10%上样量。使用Rab11FIP1 (D9D8P) Rabbit mAb进行western blot分析。 | |
Western blot analysis of extracts from MCF7 and SNB19 cells using Rab11FIP1 (D9D8P) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457.使用Rab11FIP1 (D9D8P) Rabbit mAb 和β-Actin (D6A8) Rabbit mAb #8457对MCF7和SNB19细胞提取物进行western blot分析。 |
