| 货号 | 7622S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Rabex-5 (D21F12) Rabbit mAb recognizes endogenous levels of total Rabex-5 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Rabex-5, also called RabGEF1 and RAP1, was identified as a guanine nucleotide exchange factor (GEF) for Rab5, a member of the Ras superfamily of small Rab GTPases (1). Rabex-5 generates the GTP-bound active form of Rab5 and forms a tight association with its effector protein Rabaptin-5 (2). This complex localizes to endosomal membranes where it functions as a key regulator of vesicular trafficking during early endocytosis (3,4). Rabex-5 is also monoubiquitinated and has ubiquitin ligase activity that regulates its recruitment to early endosomes (5,6). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (5). Through its control of endosomal trafficking and endocytosis, Rabex-5 has been shown to negatively regulate NGF-mediated neurite outgrowth (7) as well as FcεRI-dependent mast cell activation (8). |
| 存放说明 | -20C |
| 计算分子量 | 60-80 |
Western blot analysis of extracts from various cell lines using Rabex-5 (D21F12) Rabbit mAb.对多个细胞系抽提物使用Rabex-5 (D21F12) Rabbit mAb兔单抗进行Western blot分析。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a mouse Rabex-5 (variant 2) cDNA construct (+), using Rabex-5 (D21F12) Rabbit mAb. 对mock转染(-)或转染小鼠Rabex-5 (variant 2) cDNA载体(+)的293T细胞抽提液，使用Rabex-5 (D21F12) Rabbit mAb兔单抗进行Western blot分析。 | |
Western blot analysis of extracts from SH-SY5Y cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Rabex-5 siRNA I (+), using Rabex-5 (D21F12) Rabbit mAb #7622 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Rabex-5 (D21F12) Rabbit mAb confirms silencing of Rabex-5 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. 对转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或 SignalSilence® Rabex-5 siRNA I (+) 的SH-SY5Y细胞使用Rabex-5 (D21F12) Rabbit mAb兔单抗 #7622（上图）或α-Tubulin (11H10) Rabbit mAb兔单抗 #2125（下图）进行Western blot分析。Rabex-5 (D21F12) Rabbit mAb兔单抗证实Rabex-5的表达沉默，α-Tubulin (11H10)Rabbit mAb用作上样对照。 |
