RAD21 (D5Y8S) Rabbit mAb
货号：
12673S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 12673S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | RAD21 (D5Y8S) Rabbit mAb recognizes endogenous levels of total RAD21 protein. |
| 使用方法 | WB(1:1000)
IP (1:50) |
性能| 供应商 | CST |
| 背景 | The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
参考图片Western blot analysis of extracts from various cell lines using RAD21 (D5Y8S) Rabbit mAb.Western blot方法检测不同细胞系的提取物，使用的抗体为RAD21 (D5Y8S) Rabbit mAb。 |
