| 货号 | 8875S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination (HR). Research studies have shown that defects in both pathways are associated with human disease, including cancer (reviewed in 1). DSBs that arise during S or G2 phase are repaired via homologous recombination (HR), using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homologue of E. coli RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 (reviewed in 2,3). BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA) (4). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D) and they all appear to be required for efficient HR (5). Researchers have found that mutations in the Rad51 gene may be related to breast cancer risk (6). Some studies have implicated Rad51 as a potential marker for pancreatic cancer (7).电离辐射（IR），拟辐射化合物或DNA复制抑制因子都可能会导致DNA断裂（DSBs）。细胞通过两种不同但可能有交叉的信号通路感知并修复DSBs，非同源末端连接（NHEJ）和同源重组（HR）。研究发现两条通路发生缺陷都会导致疾病包括癌症的发生（1）。同源重组（HR）使用姐妹染色单体作为模板可以修复S或G2期的DSBs。Rad51重组酶，E.coli RecA的真核同源物，可以在包括Rad54和BRCA2在内的辅助蛋白的帮助下在单链DNA旁聚集并形成一条单纤维从而介导HR（2,3）。BRCA2结合Rad51以面向单链DNA，使其替换重复氮蛋白A(RPA)（4）。脊椎动物中已经发现了5种Rad51同源物（XRCC2, XRCC3, Rad51B, Rad51C,和 Rad51D），它们在HR过程中都是必须的（5）。研究人员发现Rad51基因突变与乳腺癌风险上升相关（6）。一些研究也指出Rad51可以作为一种潜在的胰腺癌分子标记（7）。 |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of extracts from various cell lines using Rad51 (D4B10) Rabbit mAb.多种细胞提取物进行使用Rad51 (D4B10)Rabbit mAb进行western blot分析。 | |
Immunoprecipitation of Rad51 from HeLa cell extracts using Rad51 (D4B10) Rabbit mAb (lane 2). Western blot detection was performed using the same antibody. Lane 1 is 10% input.使用Rad51 (D4B10)Rabbit mAb对HeLa细胞提取物进行免疫共沉淀实验（第2道）。同样的抗体也被用于western blot检测。第一道的上样量是10%。 |
