| 货号 | 2326S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Rap1B (36E1) Rabbit mAb detects endogenous levels of total Rap1B protein. It does not cross-react with Rap1A. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1,2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1,2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
Western blot analysis of cell extracts from various cell lines using Rap1B (36E1) Rabbit mAb. 使用Rap1B (36E1) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析不同细胞中Rap1B蛋白水平。 | |
Western blot analysis of HeLa cell lysates using Rap1B (36E1) Rabbit mAb without pre-absorption (A), or pre-incubated with Rab1A (B) and Rap1B (C) carboxy-terminal peptides respectively. The result showed that only Rap1B peptide specifically blocks the antibody binding in Western blot. 使用Rap1B (36E1) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析HeLa细胞中Rap1B蛋白水平，该抗体分别没有pre-absorption (A)或孵育Rab1A (B)和Rap1B (C)羧基端多肽。在免疫印迹（Western Blot）实验中，该结果显示仅有Rap1B多肽特异性封闭该抗体。 |
