| 货号 | 14466S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The breast cancer type 1 susceptibility protein (BRCA1) is an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of the DNA damage response and DNA repair. BRCA1 protein forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for regulation of BRCA1 protein function. The BRCA1-RAP80 complex (BRCA1 A complex) includes RAP80, BRCC36, BRE, Abraxas, and NBA1 and functions in G2/M phase checkpoint control (reviewed in 1,2). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® RAP80 siRNA #14519 (+), using RAP80 (D1T6Q) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Western blot analysis of extracts from various cell lines using RAP80 (D1T6Q) Rabbit mAb. | |
Confocal immunofluorescent analysis of 293T cells either untreated (left) or neocarzinostatin-treated (5 μg/mL; 1hr, right) using RAP80 (D1T6Q) Rabbit mAb (green). RAP80 foci become more numerous with neocarzinostatin treatment. Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). |
