| 货号 | 13610S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Retinoblastoma-like protein 2 (RBL2, p130) is the most predominant and active Rb family member found in quiescent cells. In these cells, RBL2 interacts with E2F4 and E2F5 to recruit the DP, RB-like, E2F, and MuvB protein (DREAM) complex to E2F target genes to repress transcription of multiple genes required for progression into S phase and mitosis (4-6). Hypophosphorylation of RBL2 during cellular senescence is required for maintenance of senescent cells (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Western blot analysis of extracts from various cell lines using RBL2 (D9T7M) Rabbit mAb. | |
Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® RBL2 siRNA I # 14712 (+), using RBL2 (D9T7M) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The RBL2 (D9T7M) Rabbit mAb confirms silencing of RBL2 expression, while the α-Actinin (D6F6) XP® Rabbit mAb is used as a loading control. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 1063T3 cells and either 10 µl of RBL2 (D9T7M) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse CCNE1 promoter primers, mouse CDC2 promoter primers, SimpleChIP® Mouse B-Myb Intron 2 Primers #13707, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
