| 货号 | 11922S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | RBX1 (D3J5I) Rabbit mAb recognizes endogenous levels of total RBX1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:2400) |
| 供应商 | CST |
| 背景 | RING-box protein 1 (RBX1 or ROC1) is an essential component of two distinct but structurally related E3 ubiquitin ligase complexes, the SCF complex and the CBC (VHL) complex (1). RBX1 mediates the neddylation of CUL1, which activates SCF E3 ligase by facilitating the ubiquitin transfer from E2 to substrates (2-4). The RING finger domain of RBX1 is required for ubiquitin ligation (5). Two evolutionarily conserved mammalian RBX family members, RBX1/ROC1 and RBX2/ROC2/SAG, have been identified (5). RBX1 is constitutively expressed and binds to CUL2/VHL, while stress-inducible RBX2 binds to CUL5/SOCS (6). |
| 存放说明 | -20C |
| 计算分子量 | 13 |
Western blot analysis of extracts from various cell lines using RBX1 (D3J5I) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为RBX1 (D3J5I) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using RBX1 (D3J5I) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组化分别检测在对照多肽（左）或抗原特异性多肽（右）存在的情况下石蜡包埋的人结肠癌，所使用的抗体为 RBX1 (D3J5I) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded mouse prostate using RBX1 (D3J5I) Rabbit mAb. 免疫组化检测石蜡包埋的小鼠前列腺组织，所使用的抗体为RBX1 (D3J5I) Rabbit mAb兔单抗。 |
